Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27
doi: 10.12659/MSM.893974
Figure Lengend Snippet: MiR-27a directly targets CDC27 and regulates its expression in TNBC cells. ( A ) The putative binding site between miR-27a and CDC27 and the designed mutant sequence without the pairing. ( B, C ) The relative firefly luciferase activity in HEK-293T ( B ) and MDA-MB-435 ( C ) cells co-transfected with 150 ng reporter plasmids and 50 nM miR-27a mimics. Both firefly and Renilla luciferase activities were measured 24 h after transfection and the firefly luciferase activity was normalized to the Renilla luciferase activity. ( D, E ) Western blot analysis of CDC27 protein expression in MDA-MB-435 transfected with miR-27a mimics or siCDC-27 ( D ) or transfected with antagomiR-27a or infected with CDC-27 lentiviral particles ( E ). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.
Article Snippet: CDC27 lentiviral expression vectors (without 3′-UTR region) were purchased from GENECHEM (China).
Techniques: Expressing, Binding Assay, Mutagenesis, Sequencing, Luciferase, Activity Assay, Transfection, Western Blot, Infection