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cdc27 expression vectors  (Genecopoeia)


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    Structured Review

    Genecopoeia cdc27 expression vectors
    Cdc27 Expression Vectors, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdc27+expression+vectors/pm32198345-254-23-29?v=Genecopoeia
    Average 94 stars, based on 1 article reviews
    cdc27 expression vectors - by Bioz Stars, 2026-07
    94/100 stars

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    Genechem cdc27 lentiviral expression vectors
    MiR-27a is significantly increased and <t>CDC27</t> expression is significantly decreased in TNBC cells. ( A ) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( B ) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( C ) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.
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    MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. ( A ) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( B ) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( C ) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

    doi: 10.12659/MSM.893974

    Figure Lengend Snippet: MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. ( A ) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( B ) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. ( C ) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

    Article Snippet: CDC27 lentiviral expression vectors (without 3′-UTR region) were purchased from GENECHEM (China).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    MiR-27a directly targets CDC27 and regulates its expression in TNBC cells. ( A ) The putative binding site between miR-27a and CDC27 and the designed mutant sequence without the pairing. ( B, C ) The relative firefly luciferase activity in HEK-293T ( B ) and MDA-MB-435 ( C ) cells co-transfected with 150 ng reporter plasmids and 50 nM miR-27a mimics. Both firefly and Renilla luciferase activities were measured 24 h after transfection and the firefly luciferase activity was normalized to the Renilla luciferase activity. ( D, E ) Western blot analysis of CDC27 protein expression in MDA-MB-435 transfected with miR-27a mimics or siCDC-27 ( D ) or transfected with antagomiR-27a or infected with CDC-27 lentiviral particles ( E ). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

    doi: 10.12659/MSM.893974

    Figure Lengend Snippet: MiR-27a directly targets CDC27 and regulates its expression in TNBC cells. ( A ) The putative binding site between miR-27a and CDC27 and the designed mutant sequence without the pairing. ( B, C ) The relative firefly luciferase activity in HEK-293T ( B ) and MDA-MB-435 ( C ) cells co-transfected with 150 ng reporter plasmids and 50 nM miR-27a mimics. Both firefly and Renilla luciferase activities were measured 24 h after transfection and the firefly luciferase activity was normalized to the Renilla luciferase activity. ( D, E ) Western blot analysis of CDC27 protein expression in MDA-MB-435 transfected with miR-27a mimics or siCDC-27 ( D ) or transfected with antagomiR-27a or infected with CDC-27 lentiviral particles ( E ). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

    Article Snippet: CDC27 lentiviral expression vectors (without 3′-UTR region) were purchased from GENECHEM (China).

    Techniques: Expressing, Binding Assay, Mutagenesis, Sequencing, Luciferase, Activity Assay, Transfection, Western Blot, Infection

    MiR-27a modulates radiosensitivity of TNBC cells through targeting CDC27. ( A–D ) CCK-8 assay of cell proliferation in MDA-MB-435 ( A, C ) MDA-MB-231 ( B, D ) cells after infection with CDC27 lentiviral particle with/without treatment of IR (8 Gy) ( A, B ) or transfected with 50 nM siCDC27 with/without treatment of IR (8 Gy) ( C, D ). ( E, F ) The effect of co-transfection of miR-27a and CDC27 on cell proliferation in MDA-MB-435 ( E ) MDA-MB-231 ( F ) cells with/without treatment of IR (8 Gy). ( G, H ) The effect of co-transfection of antagomiR-27a and siCDC27 on cell proliferation in MDA-MB-435 ( G ) MDA-MB-231 ( H ) cells with/without treatment of IR (8 Gy). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: MiR-27a Modulates Radiosensitivity of Triple-Negative Breast Cancer (TNBC) Cells by Targeting CDC27

    doi: 10.12659/MSM.893974

    Figure Lengend Snippet: MiR-27a modulates radiosensitivity of TNBC cells through targeting CDC27. ( A–D ) CCK-8 assay of cell proliferation in MDA-MB-435 ( A, C ) MDA-MB-231 ( B, D ) cells after infection with CDC27 lentiviral particle with/without treatment of IR (8 Gy) ( A, B ) or transfected with 50 nM siCDC27 with/without treatment of IR (8 Gy) ( C, D ). ( E, F ) The effect of co-transfection of miR-27a and CDC27 on cell proliferation in MDA-MB-435 ( E ) MDA-MB-231 ( F ) cells with/without treatment of IR (8 Gy). ( G, H ) The effect of co-transfection of antagomiR-27a and siCDC27 on cell proliferation in MDA-MB-435 ( G ) MDA-MB-231 ( H ) cells with/without treatment of IR (8 Gy). Data are shown as mean ±S.D. by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

    Article Snippet: CDC27 lentiviral expression vectors (without 3′-UTR region) were purchased from GENECHEM (China).

    Techniques: CCK-8 Assay, Infection, Transfection, Cotransfection